大腹皮对洛哌丁胺诱导的大鼠便秘的肠道菌群影响的研究

A study and an analysis of gut microbiota in the treatment of constipation with Pericarpium Arecae

中文关键词:大腹皮  便秘  16SrRNA  肠道菌群；短链脂肪酸

keywords:Pericarpium Arecae  constipation  16S rRNA  gut microbiota  short-chain fatty acids

中文摘要:[摘要]目的：本研究采用大鼠便秘模型，分析大腹皮调节肠道菌群和短链脂肪酸治疗便秘的内在机制。 方法：大鼠随机分为6组：对照组、便秘模型组、阳性药组、大腹皮高中低剂量组。采用洛哌丁胺诱导大鼠便秘模型，观察大腹皮对便秘的治疗作用。通过测量体重增长率、粪便含水量、胃肠道转运率、病理切片和胃肠激素水平评估大腹皮药理学疗效，运用16S rRNA分析大腹皮高剂量组对肠道菌群的调节作用，运用气相色谱法测定大腹皮高剂量组对肠道短链脂肪酸的影响。 结果：大腹皮不同剂量组可以缓解洛哌丁胺诱导的大鼠便秘。经治疗后显著提高血清胃动素、血管活性肠肽、P物质和乙酰胆碱的水平。16S rRNA分析显示，大腹皮可以影响g__norank_f__Eggerthellaceae, g__Candidatus_Stoquefichus, g__Lactobacillus, g__Blautia, g__Candidatus_Saccharimonas和 g__unclassified_f__Prevotellaceae菌群的相对丰度；大腹皮可以提高肠道中乙酸、丙酸和丁酸的含量。 结论:大腹皮对便秘具有良好的缓解作用，其作用机制与调节特定肠道菌群（g__norank_f__Eggerthellaceae, g__Candidatus_Stoquefichus, g__Candidatus_Saccharimonas, g__Lactobacillus, g__Blautia, g__unclassified_f__Prevotellaceae）的相对丰度和提高肠道中乙酸、丙酸和丁酸的含量相关，可能是其保护胃肠道粘膜，增强胃肠道运动的内在机制。

Abstract:Abstract: Objective: This study employs a rat constipation model to analyze the intrinsic mechanism by which Pericarpium Arecae (PA) regulates intestinal flora and short-chain fatty acids treat constipation. Methods: Rats were randomly divided into six groups: control group, constipation model group, positive drug group, and high, medium, and low-dose groups of PA. The constipation model was induced in rats using loperamide, and the therapeutic effect of PA on constipation was observed. The pharmacological efficacy of PA was evaluated by measuring body weight growth rate, fecal moisture content, gastrointestinal transit rate, pathological sections, and gastrointestinal hormone levels. The regulatory effect of the high-dose group of PA on intestinal flora was analyzed using 16S rRNA, and the effect of the high-dose group of PA on intestinal short-chain fatty acids was determined using gas chromatography. Results: Different dosage groups of PA could alleviate loperamide-induced constipation in rats. After treatment, the levels of serum motilin, vasoactive intestinal peptide, substance P, and acetylcholine were significantly increased. 16S rRNA analysis showed that PA could affect the relative abundance of g__norank_f__Eggerthellaceae, g__Candidatus_Stoquefichus, g__Lactobacillus, g__Blautia, g__Candidatus_Saccharimonas, and g__unclassified_f__Prevotellaceae bacterial communities; PA could increase the content of acetic acid, propionic acid, and butyric acid in the intestine. Conclusion: PA has a good alleviating effect on constipation, and its mechanism of action is related to regulating the abundance of specific gut microbiota (g__norank_f__Eggerthellaceae, g__Candidatus_Stoquefichus, g__Candidatus_Saccharimonas, g__Lactobacillus, g__Blautia, g__unclassified_f__Prevotellaceae) and increasing the content of acetic acid, propionic acid, and butyric acid in the intestine. This may be its intrinsic mechanism for protecting the gastrointestinal mucosa and enhancing gastrointestinal motility.