层次分析-熵权法结合响应面法优化蟾皮中蟾毒内酯类成分提取工艺

Optimization of extraction process of bufogenins in toad skin by analytic hierarchy process-entropy weight method combined with response surface method

中文关键词:蟾皮  蟾毒内酯  层次分析法  熵权法  响应面法  不同部位

keywords:toad skin  Bufadienolides  analytic hierarchy process  entropy weight method  response surface methodology  different parts

中文摘要:目的：中华大蟾蜍是传统中药材，其蟾毒内酯类成分具有抗肿瘤、抗炎等重要药理活性。优化蟾皮中蟾毒内酯类成分的提取工艺，并基于最佳工艺测定其在中华大蟾蜍不同药用部位中的含量差异。方法：以采用层次分析-熵权法计算蟾皮中 6 种成分含量的综合评分为指标，以料液比、乙醇浓度、提取时间、提取温度 4 个因素进行单因素实验，结合响应面设计筛选蟾皮的最佳提取工艺。应用所得最佳工艺条件，测定并比较中华大蟾蜍不同部位中 6 种成分的含量。结果：蟾皮提取最佳工艺条件为：乙醇浓度 87%、料液比 1:17 g/mL、提取时间为 1.3 h、提取温度为 80 ℃。蟾酥中 6 种成分的总含量远高于其他部位，蟾肝中含量最低。结论：优化的蟾皮提取工艺合理、稳定。通过对蟾蜍 7 个部位的含量测定，蟾酥中该类成分含量最高，且蟾蜍其他部位中也存在该类成分。本研究明确了蟾蜍非传统药用部位的潜在价值，对促进蟾蜍资源的综合利用与开发具有积极意义。

Abstract:Objective: The Chinese giant toad is a traditional Chinese medicinal material, and its bufadienolide components have important pharmacological activities, such as anti-tumor and anti-inflammatory effects.Optimize the extraction process of bufadienolides from toad skin, and, based on the optimal process, determine the differences in their content in different medicinal parts of the Chinese toad. Method:Using the Analytic Hierarchy Process-Entropy Weight Method to calculate the comprehensive score of the content of six components in toad skin as an indicator, a single-factor experiment was conducted with four factors: material-to-liquid ratio, ethanol concentration, extraction time, and extraction temperature, combined with response surface design to select the optimal extraction process for toad skin.Apply the optimal process conditions obtained and determine and compare the content of six components in different parts of the Chinese toad. Results:The optimum extraction conditions were as follows : ethanol concentration 87%, solid-liquid ratio 1:17 g/mL, extraction time 1.3 h, extraction temperature 80 ℃.The total content of the six components in toad venom is much higher than in other parts, with the lowest content found in the toad liver. Conclusion:The optimized toad skin extraction process is reasonable and stable.Through the measurement of the contents in seven parts of the toad, it was found that this type of component is most abundant in toad venom, and it is also present in other parts of the toad.This study highlights the potential value of non-traditional medicinal parts of toads, which is significant for promoting the comprehensive utilization and development of toad resources.